| 1. | A virus is negatively charged above its isoelectric point .
病毒在它的等电离点以上时带负电荷。 |
| 2. | This ph is termed isoelectric point , at which proteins show their minimum solubility
这个ph称为等电点，蛋白质在这一点上溶解度最小。 |
| 3. | Objective : to determine the amino acid composition and isoelectric point of porcine platelet - derived growth factor
摘要目的：测定猪血小板衍生生长因子的氨基酸组成和等电点。 |
| 4. | It also had common features of plant nsltps ( non - specific lipid transfer proteins ) such as eight conserved cysteine positions , high isoelectric points ( 8 . 9 ) and lack of tryptophans
其推测的氨基酸序列与genbank中注册的ltp蛋白前体的同源性达63 ，且具有植物lh蛋白共同特征，如8个保守的半肌氨酸位点，较高的等电点oi 8 |
| 5. | At last , we got the pure protein with the determination of electrophoresis . the molecular weight of the enzyme is estimated at 30903 through gel filtration and sds - page electrophoresis . the isoelectric point is 8 . 6 ? . 3
经sds凝胶电泳和凝胶过滤层析两种方法测定该酶的分子量分别为33113dalton和28840dalton ；其等电点pi = 8 . 6 0 . 3 。 |
| 6. | The water flux decreases with increasing ph and increases with 1 . the bsa rejection is highest at the isoelectric point ( iep ) ( 4 . 8 ) , while decreases on both sides of iep . the bsa rejection increases as i decreases
在低离子强度(高ph值)溶液中，分子链伸展，膜通量较小；而在高离子强度(低ph值)溶液中，分子链收缩，膜通量较大。 |
| 7. | Methods : in the present experiment , acid dehydroxy amino acid analysis and isoelectric focusing in polyacylamide gels were used and the amino acid composition and isoelectric point of porcine platelet - derived growth factor were studied
方法：用酸水解及聚丙烯酰胺凝胶等电聚焦的方法测定猪血小板衍生生长因子的氨基酸含量、组成成分和等电点。 |
| 8. | The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd , 120 kd and 91 kd , respectively . we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5 . 5 and 5 . 7 . immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph , which as vn was deposited in the ovary , while not in the male
Page电泳结果表明：丽蝇蛹集金小蜂明显存在2条雌特异性带-卵黄蛋白，分子量分别为181kd和136kd ； sds - page电泳分析：存在3条雌特异性带，其分子量为123kd 、 120kd和91kd ，由此，可推定卵黄原蛋白( vitellogenin ， vg )和卵黄磷蛋白( vitellin ， vn )由2个蛋白组成，其中分子量较大的蛋白由2个亚基组成；双向电泳结果显示，在120kd附近有两个特异性点，其等电点为5 . 5和5 . 7 ；双扩散表明，丽蝇蛹集金小蜂卵黄磷蛋白的抗血清与雌隐成虫虫体、脂肪体、血淋巴和卵巢匀浆液均有免疫沉淀反应，而与雄蜂血淋巴无免疫反应，说明了vg与vn具有免疫同源性，是雌特异性蛋白，且由脂肪体合成。 |
| 9. | Abstract : three theory problems about technology of leather making are discussed . the first is the difference between surface charge and isoelectric point ( pi ) . the second is about the origin of the calculation formula of basicity in making cr3 + liquor . the third is the swelling mechanism of a skin or hide in basic medium
文摘:本文讨论了皮革制造中的三个理论问题，一是表面电荷与电点( pi )之间差别，二是三价铬鞣液配制中公式的简单来源，三是生皮在碱液中膨胀的机理。 |
| 10. | Firstly , the eo genes of csfv - jl and csfv - ln9912 strains were amplified by rt - pcr and nested pcr and then sequenced after cloned into t easy vector . comparing the eo sequences with other strains eo by biosoftwares , dnastar5 . 0 and bioedit , the phylogenetic analyse revealed that all of the strains we have sequenced could be divided into groupl and groupii . two amino acid streches of 15 csfv strains eo , which form the rnase active site , and histidine residues essential for rnase catalysis in both ones were highly conserved . the eo protein propertys of antigen epitope , hydrophobicity , isoelectric point were also predicted by bioinformatic method
首先，采用rt - pcr和nest - pcr技术扩增了csfv - jl株和csfv - ln9912株eo基因，插入teasy载体后测序，应用dnastar5 . 0和bioedit软件将其与本实验室已测其它毒株和genebank中登录的csfv代表毒株eo基因序列进行比较，绘制了遗传进化树并预测了eo蛋白的抗原表位、疏水性、等电点等特性。 |
